Fig. 7: The COG1-OsSERL2 complex causes the activation of OsMAPK3 to transmit cold signal. | Nature Communications

Fig. 7: The COG1-OsSERL2 complex causes the activation of OsMAPK3 to transmit cold signal.

From: The COG1-OsSERL2 complex senses cold to trigger signaling network for chilling tolerance in japonica rice

Fig. 7

a, b, c and d The phospho-Thr194 and phospho-Tyr196 levels of OsMAPK3 (pOsMAPK3) in cog1-1 (a), in COG1 overexpression lines (COG1-OE1 and COG1-OE4) (b), in osserl2-1 (c), and in MYC-OsSERL2 overexpression lines (OsSERL2-OE1 and OE2) (d). Total protein extracts were used for immunoblotting with anti-pTEpY and anti-actin antibodies. The relative band intensities were measured with Image J. The ratio of the band intensities of pOsMAPK3 to actin in ZH11 after 4 °C treatment for 0 h was set to 1.0. Representative figures of a–d from three biological repeats. e Working model of COG1. COG1 might originated from Chinese populations of O. rufipogon and been under selection in japonica rice during domestication. The receptor like protein COG1 interacts with OsSERL2 and this interaction is enhanced by chilling. The interaction promotes activation to OsSERL2 for sensing cold signals. The COG1-OsSERL2 complex causes the activation of OsMAPK3 to transmit cold signal from the membrane to the cytoplasm, enhancing cold tolerance in rice. See also Supplementary Fig. 6. Source data are provided as a Source Data file.

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