Fig. 5: NASTIC of trajectory segments (segNASTIC).
From: Super-resolved trajectory-derived nanoclustering analysis using spatiotemporal indexing
a Schematic representation of trajectory segment thresholding based on overlap with segments from other trajectories. b Sx1a-EGFP imaged by uPAINT using Atto-647-labeled anti-GFP nanobodies in PC12 cells. Spatiotemporal clusters were identified using spatiotemporal indexing of trajectory bounding boxes using r = 1.2 and t = 20 s. Each colored cluster boundary represents the convex hull of the detections belonging to all trajectories in the cluster. c Pseudo-density map of trajectory segment overlap, with each trajectory colored according to the number of overlaps with other trajectory segments, as determined by spatiotemporal indexing of segment bounding boxes. d Spatiotemporal clusters identified using thresholded segments t = 20 s. Each colored cluster represents the convex hull of detections belonging to the clustered segments. All trajectories containing clustered segments are shown in the same color as the cluster. Source data are provided as a Source Data file.
