Fig. 4: Virulence and transmissibility of JS/LG/21-7GD in Pigs.

a Schematic representation of the gene-deleted JS/LG/21-7GD. The deleted gene segments were replaced with the p72mCherry and p72Venus reporter gene cassettes as indicated, respectively. Nucleotide positions indicating the boundaries of the deletion relative to the ASFV JS/LG/21 genome are indicated. b Growth curve of JS/LG/21 and JS/LG/21-7GD in PAMs. PAMs were infected with JS/LG/21 and JS/LG/21-7GD at an MOI of 0.1, respectively. The supernatants were collected from three wells for viral DNA detection using qPCR at the indicated timepoints. Data were presented as mean values ± SD. c Fluorescence of PAMs infected with JS/LG/21-7GD at 72 h post-inoculation. This experiment was performed three times and the data from one independent experiment were shown. Ten SPF pigs were inoculated with 10⁶ TCID₅₀ of JS/LG/21-7GD. Rectal temperature (d) and survival (e) were monitored daily for 28 days after infection. f Sera were collected from ten inoculated pigs at the indicated timepoints for ASFV-specific antibody detection using a blocking ELISA kit (in-house). Data were presented as mean values ± SD. The dashed black lines indicate the normal rectal temperature (40 °C) of pigs. Source data are provided as a Source Data file.