Fig. 10: Interactions between the Acheta domesticus densovirus (AdSDV) ssDNA genome and the capsid interior.

a Interior view of the AdSDV twofold axis, with the representation of electron density zoned to the six ordered nucleic acid bases (ơ=3), only present in case of the genome-filled full capsids (FC). The ribbon diagram representation of the chain A atomic model is shown in blue and its twofold-neighboring subunit in black. The fivefold-neighboring subunit of chain A is presented in green, while the fivefold- neighbor of its twofold neighboring subunit in yellow. The atomic model of the nucleic acid is shown in cyan. b Side cross section view of the β-annulus, occupying the AdSDV threefold axis. The actual threefold axis is marked by an arrow. Electron density is shown zoned to the final C-terminally ordered residues, indicating that the ordered portion of the FC C-terminus stretches far and bends underneath the twofold symmetry axis. In the absence of a packaged genome, the ordered region ends directly underneath the threefold axis. c The AdSDV genome interacts with the interior surface via π-stacking interactions, underneath the twofold symmetry axis. Note how these stacks are the result of interactions between two ssDNA regions and four subunits, colored the same as in a. d Cros-section of the AdSDV FC map, with density shown as a mash at ơ=1.5. The closer the interior genome density is located to the twofold symmetry axis, the more ordered it appears. The cyan arrow points to the location of the ordered nucleotides under the twofold symmetry axis. e Melting temperature profile of the AdSDV capsids and VLPs at the four pHs parvoviruses may encounter during endo-lysosomal trafficking, performed in duplicates. Note the difference in thermostability between the empty particles and VLPs (EC1, EC2, EC, vpORF1-VLPs) vs. the genome-containing FC population. Results of n = 2 independent runs are shown. Source data are provided as a Source Data file for this panel.