Fig. 5: Cryo-EM single particle structural studies of the Acheta domesticus segmented densovirus (AdSDV) capsids and vpORF1 virus-like particles (VLPs).

a Surface (left) and cross-section (right) views of the obtained AdSDV capsid and vpORF1 VLPs electron density maps, rendered at ơ=1, with the example density and atomic model shown next to each map at ơ = 4. The maps are colored radially, orientated in the I1 icosahedral convention (twofold axis in z plane). The five- three- and twofold symmetry axes are marked by a pentagon, triangle, and ellipse, respectively. The full, genome containing capsid fraction is labeled FC, while EC abbreviates the various empty capsid fractions pulled from the sucrose and isopycnic CsCl gradients. b Ribbon diagram showing the atomic model of a single subunit from the AdSDV full capsid (FC). Structurally conserved elements are shown in black and gray, the loops connecting these are highlighted in their respective colors. Symmetry axes are labeled the same as in panel A, to indicate orientation. c Superimposition of the subunit atomic model ribbon diagrams, obtained from the genome-filled infectious capsid population (FC), from the empty capsid fraction with the highest-buoyancy (EC1) and the vpORF1, recombinantly expressed VLPs.