Fig. 2: Storage and retrieval of multiple images in a single complex pool.
From: A biological camera that captures and stores images directly into DNA
Red boxes indicate wells with allocated bits that do not correspond with the projected image. A Projected patterns that are stored with the BacCam process. Images are projected on different plates, barcoded individually with index sequences corresponding to each image, and resulting products are pooled together into a single tube. B Retrieved images from a single sequencing run of a mixed pool, deconvoluted with the corresponding indexes of each image. C Retrieved images from multiple sequencing runs, whereby random access of each image was conducted from the mixed pool by using specific primers to amplify desired images before sequencing. D Reconstruction of selectively amplified single images from mixed libraries of increasing dilutions, demonstrating robustness of the amplification technique in selecting specific images from an ever-decreasing initial amount of DNA. Source data are provided as a Source Data file.
