Fig. 1: Effect of PIP2 on enNTS1ΔM4 13CεH3-methionine chemical shifts. | Nature Communications

Fig. 1: Effect of PIP2 on enNTS1ΔM4 13CεH3-methionine chemical shifts.

From: Stabilization of pre-existing neurotensin receptor conformational states by β-arrestin-1 and the biased allosteric modulator ML314

Fig. 1: Effect of PIP2 on enNTS1ΔM4 13CεH3-methionine chemical shifts.The alternative text for this image may have been generated using AI.

a Cartoon representation of thermostabilized rNTS1 (PDB 4BWB) with labelled methionine methyl groups shown as yellow spheres (superscript - Ballesteros-Weinstein nomenclature41) and NT8-13 shown as purple sticks. Overlays of Apo-state (b), NT8-13 (c), ML314 (d), and NT8-13 & ML314 (e) bound 1H-13C HMQC spectra in the absence and presence of 130 μM (2x molecular equivalents over enNTS1) PIP2. The corresponding peak intensities are plotted in Supplemental Fig. S1. All spectra were recorded at 600 MHz, in 3 mm thin wall precision NMR tubes (Wilmad), with enNTS1ΔM4 concentrations of 66 μM.

Back to article page