Fig. 5: Comparison of Gαiq:enNTS1ΔM4 and βArr1-3A:enNTS1ΔM4 ternary complex NMR spectra. | Nature Communications

Fig. 5: Comparison of Gαiq:enNTS1ΔM4 and βArr1-3A:enNTS1ΔM4 ternary complex NMR spectra.

From: Stabilization of pre-existing neurotensin receptor conformational states by β-arrestin-1 and the biased allosteric modulator ML314

Fig. 5: Comparison of Gαiq:enNTS1ΔM4 and βArr1-3A:enNTS1ΔM4 ternary complex NMR spectra.The alternative text for this image may have been generated using AI.

a Overlay of NT8-13:enNTS1ΔM4:βArr1-3A (purple) and NT8-13:enNTS1ΔM4:Gαiq (tomato) 1H-13C HMQC spectra. Peaks marked with an asterisk represent natural abundance βArr1-3A and Gαiq resonances (Supplementary Figs. 2b and 6a). b Overlay of NT8-13:ML314:enNTS1ΔM4:βArr1-3A (royal blue) and NT8-13:ML314:enNTS1ΔM4:Gαiq (medium purple) 1H-13C HMQC spectra. Peaks marked with an asterisk represent natural abundance βArr1-3A and Gαiq resonances. Extracted spectra regions of enNTS1ΔM4:Gαiq and enNTS1ΔM4:βArr1-3A methionine resonances in the presence of (c) NT8-13 and (d) NT8-13:ML314. One dimensional 1H cross-sectional slices (corresponding to dashed lines in 2D spectra) shown on top. Dots denote the residue’s chemical shift position in spectra of the corresponding color with additional dots shown for ligand-only spectra (NT8-13:enNTS1ΔM4, forest green; NT8-13:ML314:enNTS1ΔM4, maroon). Gαiq containing spectra were recorded at 600 MHz with receptor concentrations of 64 μM and βArr1-3A containing spectra with receptor concentration of 66 μM.

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