Fig. 2: Association between structural avidity and tumor tropism.

a Correlation between the structural profile of antigen-specific CD8 T cells and tropism at steady state. b Representative examples and cumulative analyses (Mean ± SEM) of monomeric pMHC-TCR dissociation kinetics of MMP9-specific PBLs and TILs assessed with NTAmers (reversible pMHC multimers). The number of clones is indicated in brackets (each clone was tested individually). P values are provided at 95% confidence interval and using two-sided Mann–Whitney test. c Comparison of the structural avidity of seven pairs of PBLs and TILs recognizing the same pMHCs. The number of clones is indicated in brackets (each clone was tested individually). Wilcoxon two-sided test was used to determine the P value. d Structural avidity of TAA- and neoantigen-specific PBLs and TILs. The number of clones is indicated in brackets. P values are provided at 95% confidence interval and using two-sided Mann–Whitney test when significant. e UTP20-specific CD8 T cells from patient Lung1 were sorted from TILs and PBLs using NTAmers, bulk TCR sequenced and cloned by limiting dilution. The Manhattan plots of TCRα repertoires are shown and only clonotypes identified in both PBLs and TILs repertoires are color-coded. f Monomeric pMHC-TCR dissociation kinetics of three UTP20-specific clones of patient Lung1 assessed with reversible pMHC multimers (NTAmers). g Relative frequency of clones 1, 3 and 5 among UTP20-specific CD8 TILs (left) and PBLs (right). Structural avidity for each clone is also plotted. h Superimposition of in silico analyses of the pMHC-TCRs molecular interactions for UTP20-specific clones 5 and 1. Pink and green are used to color TCR ribbons, MHC (shaded color) and peptides (ball and stick) for clones 5 and 1, respectively. Source data are provided as a Source Data file.