Fig. 5: Epigenetic regulation of ATE-G isoforms in the GER5 locus and the impact on environmental responses and RNA stability. | Nature Communications

Fig. 5: Epigenetic regulation of ATE-G isoforms in the GER5 locus and the impact on environmental responses and RNA stability.

From: Long-read direct RNA sequencing reveals epigenetic regulation of chimeric gene-transposon transcripts in Arabidopsis thaliana

Fig. 5

a GER5-ATCOPIA78/ONSEN locus. TEs, Araport11 gene annotation, and DRS-AtRTD3 transcript isoforms are shown. * indicates isoforms examined by RT-qPCR in c. b, c Relative expression of transcripts corresponding to GER5 protein coding sequence (CDS; AT5G13200.1 and AT5G13200.2) and ATE-G isoform (MSTRG.3521.2, 4, 6) under mock and ABA stress conditions in indicated genotypes. Bars represent the means of four biological replicates ± standard error of the mean (SEM). *, p < 0.05 by t-test for comparison between Col-0 and mutants under mock conditions, and +, p < 0.05 by t-test under ABA stress conditions. d Relative expression of transcripts corresponding to GER5 CDS (AT5G13200.1) in the A. thaliana ecotypes with or without ATCOPIA78/ONSEN insertion in the 3′-UTR. Bars represent the means of four biological replicates ± SEM. *, p < 0.05 by t-test for comparison between Col-0 and mutants. e Relative transcript levels of GER5 (AT5G13200.2) at 0, 30, 60, 90, and 120 min after cordycepin treatment in Col-0, ibm2, edm2, suvh456, and ecotypes without ATCOPIA78/ONSEN insertion (Ler-0 and Sha). Expression levels at 0 min are set as 1. Bars represent the means of four biological replicates ± SEM. *, p < 0.05 by t-test. Source data are provided as a Source Data file.

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