Fig. 9: Independent triggering of cell cycle start and mitosis to simulate wild-type timing. | Nature Communications

Fig. 9: Independent triggering of cell cycle start and mitosis to simulate wild-type timing.

From: Multidimensional characterization of inducible promoters and a highly light-sensitive LOV-transcription factor

Fig. 9

A Illustration of the protocol. B Budding-to-anaphase duration with 20% diascopic light intensity. C Budding-to-anaphase duration with 80% light intensity. cln∆* denotes cln∆ MET3pr-CLN2pr while clb1,2∆* denotes clb1,2∆ GALL-CLB2. LIP-CLB2kd stands for LIP-CLB2kd-yEVenus. The same experiment with control cln∆* clb1,2∆* cells (without the LIP-CLB2kd-yEVenus construct) is shown in (B, C). Number of scored cells shown in Supplementary Table 18.

Back to article page