Table 1 Meta-analysis of proteins that remain soluble and misfolded in the presence of chaperones using Eq. 1. See also Supplementary Table 1
From: How soluble misfolded proteins bypass chaperones at the molecular level
Protein name | Chaperone(s) | %Misfolded and soluble (a1 in Eq. 1) | Slow folding time constant, (min) | k2 (min−1) | k2, Upper bound | k2, Lower bound | Reference number |
|---|---|---|---|---|---|---|---|
Aconitase | GroEL | 57 | 103 | 2.18 × 10−3 | 2.22 × 10−3 | 2.14 × 10−3 | 5 |
Peptidase Q* | GroEL | 19 | 104 | 1.65 × 10−4 | 0.36 × 10−2 | 1.9 × 10−23 | 6 |
Luciferase* | HSP70/DnaK | 8 | 1022 | 9.34 × 10−22 | 2.55 × 10−14 | 4.67 × 10−22 | 7 |
Luciferase | HSP33 | 43 | 1016 | 1.71 × 10−16 | 1.72 × 10−16 | 1.39 × 10−33 | 8 |
Rhodanese | GroEL, Dnak, GrpE | 26 | 1016 | 1.80 × 10−16 | 3.74 × 10−20 | 1.21 × 10−16 | 9 |
Malate dehydrogenase* | Hdea | 50 | 1018 | 4.90 × 10−18 | 4.62 × 10−4 | 4.54 × 10−22 | 10 |
Luciferase | HSP70, HSP90 | 35 | 102 | 1.50 × 10−2 | 1.58 × 10−2 | 1.43 × 10−2 | 11 |
Rhodanese* | HSP60 | 26 | 1019 | 1.06 × 10−19 | 2.21 × 10−19 | 5.31 × 10−20 | 12 |
Malate dehydrogenase* | cpn60 | 27 | 1023 | 5.62 × 10−23 | 1.11 × 10−3 | 2.81 × 10−23 | 13 |
Rhodanese* | GroEL | 17 | 103 | 9.80 × 10−3 | 4.40 × 10−2 | 2.06 × 10−20 | 14 |
Rubisco† | GroEL | 38 | 102 | 7.80 × 10−2 | 8.18 × 10−2 | 7.44 × 10−2 | 15 |
Rubisco | GroEL | 70 | 1020 | 4.82 × 10−20 | 2.85 × 10−14 | 7.27 × 10−19 | 16 |
Luciferase | HSP70, HSP90 | 40 | 1019 | 9.85 × 10−19 | 4.58 × 10−16 | 6.46 × 10−19 | 17 |
Rubisco* | GroEL | 10 | 1012 | 1.01 × 10−12 | 9.72 × 10−3 | 5.95 × 10−26 | 18 |
Rubisco | GroEL | 23 | 1023 | 2.07 × 10−23 | 3.58 × 10−13 | 1.03. × 10−23 | 19 |
Interferon | GroEL | 25 | 103 | 7.23 × 10−3 | 7.71 × 10−3 | 6.80 × 10−3 | 20 |
Galactosidase | HSP70/DnaK | 43 | 1022 | 2.19 × 10−22 | 7.75 × 10−15 | 1.09 × 10−22 | 21 |
Rubisco | GroEL | 14 | 1014 | 3.14 × 10−14 | 4.73 × 10−13 | 1.90 × 10−25 | 22 |
Malate dehydrogenase | GroEL | 6 | 1013 | 1.39 × 10−13 | 3.19 × 10−13 | 6.96 × 10−21 | 23 |
Rhodanese | GroEL | 20 | 1020 | 9.34 × 10−16 | 2.92 × 10−20 | 1.97 × 10−20 | 24 |
