Fig. 4: CFP1 is required for the counteraction of P4 on E2-dependent uterine growth in a mouse model of human endometriosis. | Nature Communications

Fig. 4: CFP1 is required for the counteraction of P4 on E2-dependent uterine growth in a mouse model of human endometriosis.

From: CFP1 governs uterine epigenetic landscapes to intervene in progesterone responses for uterine physiology and suppression of endometriosis

Fig. 4

a Schematic protocol to induce an experimental model of human endometriosis with transplantation of Cfp1f/f or Cfp1d/d uterine fragments. b–e Representative photographs to show the in vivo locations (b) and size (c) of ectopic lesions (dashed circles) harvested 15 days after transplantation of small pieces of Cfp1f/f and Cfp1d/d uterine tissues in recipients treated with E2, E2 + P4 + PBS, or E2 + P4 + SAG. Scale bar, 5 mm. Average volumes (d) and numbers (e) of ectopic lesions collected from recipients. n = 5 to 6 biologically independent samples per group. Statistical analyses were performed using the multiple comparisons. *p < 0.05, **p < 0.01. f Real-time RT-PCR analyses of Cfp1, Gata2, Sox17, and Ihh expression in Cfp1f/f and Cfp1d/d ectopic uterine lesions in recipients treated with E2 and P4. n = 3 biologically independent samples per genotype. Data are presented as mean values with SD. Statistical analyses were performed using the unpaired Student’s t-tests. *p < 0.05, **p < 0.01.

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