Fig. 2: YAP/TAZ modulates erectile function and facilitates restoration in ED model mice.
From: The role of mechano-regulated YAP/TAZ in erectile dysfunction
a ICP from wild-type (Control, n = 5), Myh11CreERT2; Yapfl/+; Tazfl/fl (Taz cKO, n = 10), Myh11CreERT2; Yapfl/fl; Tazfl/+ (Yap cKO, n = 6) and Myh11CreERT2; Yapfl/fl; Tazfl/fl (Y/T cKO, n = 5) mice. b ICP from the control (n = 6) and verteporfin-treated (n = 7) mice. c Immunohistochemistry images of TAZ from control and ED patients, each image represents a human sample, the experiments were repeated three independent times with similar results. d ICP (upper panel) and immunofluorescence images (bottom panel) of YAP/TAZ from sham rats and the indicated time points of the BCNI-induced rat ED models. e Quantification of the ICP in sham rats (n = 12) and 5 days (n = 4), 14 days (n = 4), 28 days (n = 5) and 60 days (n = 4) indicated time points of the BCNI-induced rat ED models. f Quantification of immunofluorescence images of YAP/TAZ (red), αSMA (green) and DAPI (blue) from sham rats (n = 3) and 5 days (n = 5), 14 days (n = 3), 28 days (n = 3) and 60 days (n = 3) indicated time points of the BCNI-induced rat ED models. g Expression of the YAP/TAZ target gene Ctgf from sham rats (n = 6) and 5 days (n = 6), 14 days (n = 4), 28 days (n = 4) and 60 days (n = 5) indicated time points of the BCNI-induced rat ED models. Similar results were obtained by checking Cyr61 expression (Supplementary Fig. 2c). h ICP (upper panel) and immunofluorescence images (bottom panel) of YAP/TAZ from the control and indicated time points of the IR-induced rat ED models. i Quantification of the ICP in control (n = 10) and 1 month (n = 4), 2 months (n = 4), 3 months (n = 5) and 5 months (n = 6) indicated time points of the IR-induced rat ED models. j Quantification of immunofluorescence images of YAP/TAZ (red), αSMA (green) and DAPI (blue) from control (n = 6) and 1 month (n = 3), 2 months (n = 4), 3 months (n = 4) and 5 months (n = 3) indicated time points of the IR-induced rat ED models. k Expression of the YAP/TAZ target gene Ctgf from control (n = 15) and 1 month (n = 5), 2 months (n = 5), 3 months (n = 5) and 5 months (n = 5) indicated time points of the IR-induced rat ED models. Similar results were obtained by checking Cyr61 expression (Supplementary Fig. 2h). l ICP and quantifications from sham (n = 8) and castration (n = 4) mice. m Immunohistochemistry images of TAZ from sham and castration mice, the experiments were repeated three independent times with similar results. n Upper panel, schematics of the corresponding experimental design. Representative images (bottom) and quantifications (right) of the ICP from the sham- (n = 8), BCNI- (n = 11), and verteporfin-treated (n = 6) BCNI mice. Box plots indicate median (middle line), 25th, 75th percentile (box), minima, maxima and all points. Dot plots indicate mean (middle line) and all points. Bar charts are presented as the mean ± sem. The statistical analysis was calculated by two-sides unpaired Student’s t test, the confidence interval is 95%. Each point represents a mouse or rat biologically independent sample. Scale bars, 10 µm. Source data are provided as a Source Data file.
