Fig. 2: Spatial dynamics of cell types during regeneration.

a Dynamic change of each cell population over the scRNA-seq of whole-body regeneration time courses. Dynamic change of each cell population over the scRNA-seq of whole-body regeneration time courses. We performed five times of 20% downsampling, and the standard deviation was shown as the error bands. Source data are provided as a Source Data file. b Spatial change of major cell types during the regeneration time courses. ST spot is illustrated as a pie chart of major cell types. c Average prediction scores and spatial enrichment test of major cell types along the anterior-posterior axis of planarian during the regeneration. Hypergeometric test (one-sided), p value <0.05*. Source data are provided as a Source Data file. d Volcano plot showing spatial differential genes that are up- and down-regulated in neoblast, neuronal, muscle, and parenchymal clusters in wound region at the A-wound and P-wound, anterior wound and posterior wound. Key regulatory genes are indicated in red, while other genes are indicated in gray. Source data are provided as a Source Data file. e Tissue sections of the anterior wound area showing the cell type with the maximum prediction score of ST spots, and prediction scores of neoblast, neuronal, and parenchymal of ST spots. f Chord diagram showing predicted signal correlation of pairwise cell types in ST spots in all regeneration time points. g Schematic representation showing the communication between neoblast and other cell types in planarian. h Spatial colocalization degree of ligand-receptor pairs bmp7-acvr1 and ncam1-fgfr1 before 24 hpa. Source data are provided as a Source Data file. i Dotplot of ligand-receptor pair NCAM1-FGFR1 showing the average expression in major cell types. j Co-expression pattern of ligand-receptor pair ncam1-fgfr1 at 0, 12, and 24 hpa respectively. See also Supplementary Fig. 5.