Fig. 4: Spatial module can help to screen for regeneration-related genes.

a Correlation heatmap of functional gene modules identified by Hotspot analysis in 12 hpa sample. Each row and each column represent a module marker gene, and Z-score indicates the correlation between module marker genes. Right panel: GO analysis showing the top function terms of each module. b The expression pattern of each module identified in (a). The “#number” next to the sections represents the number of each section. c The gene-regulated network of anterior polarity gene modules. d The expression levels of posterior polarity genes, ftm, mboat2, and smed29842, in 0 and 12 hpa ST data. e Spatial distribution of wound-induced genes egr2, traf3, and runt-1 at 0 and 12 hpa. f WISH staining of polar genes, hsp12 and smed5347, identified through the polarity gene module. Scale bar, 500 μm. See also Supplementary Fig. 8.