Fig. 6: plk1 is essential for planarian regeneration.

a Overview of study design for plk1 (RNAi) single-cell atlas of the planarian. b UMAP visualization of control and plk1 knockdown scRNA-seq samples at 5 dpa. c The location of neoblasts, progenitor, and differentiated cell clusters was shown on the UAMP plot. d Percentage of cells allocated to different cell types in control and plk1 knockdown planarians at 5 dpa. Source data are provided as a Source Data file. e FISH showing the expressions of smedwi-1, cca4, mGAT, and soxP-3 in the control and plk1 knockdown planarian at 5 dpa. Scale bar, 300 μm and 20 µm in the enlarged field. f The expression pattern of plk1 at 24 hpa and 3 dpa during regeneration. n (24 hpa from top to bottom) = 463, 1426, 1885, 1999, 1940, 1719, 481; n (3 dpa from top to bottom) = 373, 1084, 1418, 1867, 2069, 1671, 740. The middle lines of the boxes represent the medians of datasets (50th percentile). The upper and bottom lines of the boxes are, respectively, the upper quantile (25th percentile) and the lower quantile (75th percentile) of the data. The whiskers mark the upper and lower limits of these datasets. g Dotplot showing the expression of differentially expressed genes and the expression of genes with expression patterns similar to plk1, in different cell types. h The gene expression score and cell type distribution at 3 dpa. The genes with a expression pattern similar to plk1 was detected by Hotspot, and the cell type distribution were predicted by markers. i FISH showing co-expressions of soxP-3 and plk1 at 5 dpa. Scale bar, 200 μm and 20 µm in the enlarged field. Quantification of the percentage of soxP-3⁺ / plk1⁺ cells mm² of whole tissue fragment and near-wound area. Error bars represent standard deviation. Data were the mean ± SD. and n = 8 animals in each group. The p values were determined using a two-sided unpaired Student’s t-test (right). Source data are provided as a Source Data file. See also Supplementary Fig. 10.