Fig. 4: Antibody repertoire in patient’s matched blood and tumor. | Nature Communications

Fig. 4: Antibody repertoire in patient’s matched blood and tumor.

From: B cell profiles, antibody repertoire and reactivity reveal dysregulated responses with autoimmune features in melanoma

Fig. 4: Antibody repertoire in patient’s matched blood and tumor.The alternative text for this image may have been generated using AI.

a Pie charts representing the isotype distribution of the heavy and light chain sequences isolated from blood and tumor of a patient with melanoma. b Frequency distribution of heavy chain IGHV, IGHD and IGHJ genes in blood and tumor. c Frequency distribution of kappa light chain IGKV and IGKJ genes and lambda light chain IGLV and IGLJ genes in blood and tumor. d Bubble plot representing the distribution of heavy chain IGHV-IGHJ gene pairing in melanoma patient blood and tumor. e Heatmap representing IGHV gene distribution grouped by isotype, in blood and tumor; tumor-specific IGHV-isotype combinations are highlighted in red. f Unrooted circular trees showing variable domain clustering according to sequence variability in blood and tumor heavy and light chain sequences annotated with information regarding the isotype or indicated if unproductive. Error bars represent bootstrapped 95% confidence intervals; statistical significance was calculated with a two-sample proportion z-test (b–d); P < 0.05 are reported on the graphs. * = P < 0.05, ** = P < 0.01. Source data are provided as a Source data file.

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