Fig. 8: Intrathecal Gal-3 exerts a pro-nociceptive effect that requires TLR4 expression, and Gal-3 inhibitor attenuates K/BxN ST allodynia in WT but not TASTPM mice. | Nature Communications

Fig. 8: Intrathecal Gal-3 exerts a pro-nociceptive effect that requires TLR4 expression, and Gal-3 inhibitor attenuates K/BxN ST allodynia in WT but not TASTPM mice.

From: Galectin-3 activates spinal microglia to induce inflammatory nociception in wild type but not in mice modelling Alzheimer’s disease

Fig. 8

a Gal-3 (170 ng/ 5 μl; 1 μM) intrathecal injection in WT and TLR4 KO followed by behaviour at 30 and 60 min after injection. b Exogenous Gal-3-induced mechanical hypersensitivity in WT but not TLR4 KO mice. Data represent mean ± SEM, n = 8 (TLR4 KO, 4 per sex), n = 12 (WT, 6 per sex). *p < 0.05, ****p < 0.0001, ††††p < 0.0001. Two-way RM ANOVA, Tukey’s multiple comparisons test. c Intrathecal injections of GB1107 (10 nM) or 0.1% DMSO performed at Day 5 K/BxN and behaviour at 3 h after injection. d Reversal of mechanical hypersensitivity by GB1107 in WT but not TASTPM K/BxN ST. Data represent mean ± SEM, n = 8 mice per group. ***p < 0.001 WT K/BxN ST vs. WT Control ST, †p < 0.05 TASTPM K/BxN ST vs. same-day WT K/BxN ST, ##p < 0.01. GB1107− WT KBxN ST vs. DMSO− WT K/BxN ST, two-way RM ANOVA, Tukey’s multiple comparisons test.

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