Fig. 3: Optogenetic inhibition of the vmPFC during the OF task.
From: Ventromedial prefrontal neurons represent self-states shaped by vicarious fear in male mice
a Left, schematic representation of the optogenetic manipulation of the vmPFC. Right, coronal vmPFC section image stained with anti-GFP (green, for eYFP) and DAPI (4′,6-diamidino-2- phenylindole, blue). b Representative in vivo unit recording of optogenetic inhibition. c Freezing rate of the observer and the demonstrator (two-way repeated-measures ANOVA). Green line indicates the light manipulation. d, e Positioning points of the control (n = 15 mice, light blue dots) and inhibition (n = 17 mice, pink dots) data on the t-SNE atlas obtained from the previous experiment (Fig. 1d, gray dots). f Behavioral sequence of each mouse during the OF task sorted by the number of transitions within each group. g Proportion of each behavioral cluster in the habituation and conditioning periods (the permutation test, two-sided with Bonferroni correction, P < 0.025/8 = 0.0031; STAR Methods). h Proportion of each behavioral component in the habituation and conditioning periods. i Proportion of components i1 of the control and inhibition group in the habituation and conditioning periods (control: n = 15 mice, inhibition: n = 17 mice, unpaired t-test, two-sided). j Proportion of components i2 + i3 of the control and inhibition group in the habituation and conditioning periods (control: n = 15 mice, inhibition: n = 17 mice, unpaired t-test, two-sided). k The distance from the demonstrator side. The x-coordinate of the back center of the control and inhibition groups (two-way repeated-measures ANOVA). *P < 0.05, n.s. not significant. Data are presented as mean ± SEM (error bars and shadows). See Supplementary Information for exact p values. Source data are provided as a Source Data file.
