Fig. 4: Mouse liver organ-to-nucleus imaging with A-ha copolymer.
From: Transparent tissue in solid state for solvent-free and antifade 3D imaging
a, b A-ha-based tissue clearing of 500-µm-thick mouse liver (representative images of three livers). The increase in transmittance enables in-depth fluorescence imaging of sympathetic nerves (anti-tyrosine hydroxylase stain, green), blood vessels (perfusion labeling, magenta), and nuclei (DAPI stain, white) shown in c–j. c–j Representative 2D images and 3D projections of mouse liver neurovascular network. Arrows in e–g and j indicate the same bifurcation of portal vein. h–j show the subcellular features of nerve bundle and endothelium preserved in the A-ha copolymer. In panel j, green and white arrowheads indicate the perivascular sympathetic nerve fiber and elongated endothelial nuclei, respectively. Transmitted light signals of nuclei (#1 and #2) embedded in A-ha are magnified in inset (bar, 2 µm).
