Fig. 10: BA.1-LAV and BA.5-LAV as boosters. | Nature Communications

Fig. 10: BA.1-LAV and BA.5-LAV as boosters.

From: Intranasal or airborne transmission-mediated delivery of an attenuated SARS-CoV-2 protects Syrian hamsters against new variants

Fig. 10: BA.1-LAV and BA.5-LAV as boosters.The alternative text for this image may have been generated using AI.

a Genome organization of BA.1-LAV and BA.5-LAV. Leader sequence (red), transcriptional regulatory sequence within the leader sequence and within the body are highlighted as green bars. The polybasic insert “HRRA” was removed from the spike protein proteins and ORF6-8 were removed from the WA1/2020 backbone. Locations of K164A/H165A are highlighted in the figure. b Pseudovirus (PsV) bearing BA.5 spike but not WA1/2020 spike infected 293-mACE2 cell line. Infected cells are shown in green. Blue, DAPI strained nuclei. Scale bars in the lower right corner indicate 50 µm. c Overall study design to test BA.1-LAV and BA.5-LAV as boosters. 10-week-old male BALB/c mice were employed in this study. d Neutralizing antibody titers were measured from pre- and post-boost sera in BA.1 and BA.5 boosted mice (n = 5 in each group). Each solid circle represents one animal. Numbers above each group and average bars indicate the geometric means of neutralizing antibody titers with error bars signifying standard deviations. Values were compared using Student’s unpaired t test in GraphPad Prism 9.4.0.

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