Fig. 4: OSCAR-1 and OSCAR-3 efficiently control tumor growth in xenograft orthotopic OS model.

a Experimental overview of OHS orthotopic OS model. NSG mice were engrafted with GFP/Luc⁺ OHS cells implanted within the tibiae (intra-tibial, i.t.). Mice received two doses of OSCAR-1, OSCAR-3, or mock T cells and were sacrificed at day 28 (X). b Tumor burden measured by IVIS. Representative BLI images of the luminescent signal from each treatment group. c Quantification of tumor progression as mean ± s.d. per treatment group. Statistical comparisons were performed with one-way ANOVA test with Tukey’s multiple comparison test (ns not significant p > 0.05, **p < 0.01). Representative experiment is shown (OSCAR-1 n = 6 mice, OSCAR-3 n = 6 mice, mock n = 4 mice). d Detection of OSCAR-1, OSCAR-3, and mock T cells in peripheral blood (PB), spleen (SPL), intra tibia (IT) and collateral tibia (CL) from mice sacrificed on day 28 (n = 4 per group). Data are presented as mean ± s.d. Statistical comparisons were performed with one-way ANOVA test with Tukey’s multiple comparison test (ns not significant P > 0.05).