Fig. 5: Cleavage of Huh7 miRNA samples using p19-T111BpyAla.

a Schematic representation of the workflow for small RNA isolation for Nanostring profiling and RT-qPCR analysis. RNA used was isolated from Huh7 hepatocellular carcinoma cell line and treated in the presence of CuSO₄. Created with BioRender.com. b A heat map depicting some of the significant downregulated fold changes in miRNAs upon treatment with p19-T111BpyAla relative to p19-WT treated miRNAs, n = 2, n represents 2 biological trials, where RNA is isolated from 2 independent cell passages. miRNAs with counts below 20 were eliminated from the heat map. Changes presented are more than 1.5-fold change. c qRT-PCR analysis of the relative miR-122 expression in p19-T111BpyAla treated samples in comparison to that treated with p19-WT in the presence of copper ions. Error bars represent the mean ± SD, n = 3, n values represent the treatment of RNA isolated from 3 different passages. d qRT-PCR analysis of the relative miR-122 expression in p19-T111BpyAla treated samples in the presence and absence of copper ions. Error bars represent the mean ± SD, n = 3, n values represent the treatment of RNA isolated from 3 different passages. e Derepression of miR-122’s downstream direct target STAT3 upon treatment with p19-T111BpyAla, Error bars represent the mean ± SD, n = 3. n values represent the transfection of cells from 3 different passages. Unpaired two-tailed t-test was used to evaluate statistical significance.