Fig. 6: p19-T111BpyAla effect on HCV replication.
From: An unnatural enzyme with endonuclease activity towards small non-coding RNAs
a Schematic representation of the subgenomic HCV replicon (pFK-I389neo/luc/NS3-3′/5.1) used in this study52 and HCV JFH1T strain. Created using BioRender.com. b Relative luciferase signal upon treatment of E9 cells with either miR-122 antagomir, c p19-T111BpyAla or p19-WT. The levels of miR-122 are directly correlated with HCV replication, which is linked to the relative luciferase signal in E9 cells. The effects of both miR-122 antagomir, and p19-T111BpyAla were investigated, with both treatments leading to a similar reduction in HCV subgenomic replication. For b and c, n = 3, and error bars represent mean values ± SD, p-values are presented. Unpaired two-tailed t-test was used to evaluate statistical significance. d Relative expression of HCV JFH1 IRES in Huh7.5 cells upon treatment with 1 µM p19-T111BpyAla, n = 3, error bars represent mean ± SD. P-values are presented in the figure. n values represent transfections of different cell passages. Control represents no treatment. Unpaired two-tailed t-test was used to evaluate statistical significance.
