Fig. 1: The Letmd1 gene encodes a brown adipocyte-enriched and cold-inducible protein.
a mRNA expression of Letmd1 during brown adipocyte (iBPA) differentiation. Expression is normalized to Rpl32. n = 3 per group. b Western blots of LETMD1 and UCP1 proteins during brown adipocyte (iBPA) differentiation. HSP90 is a loading control. c Western blot of LETMD1 protein in brown adipose tissue (BAT), inguinal white adipose tissue (iWAT), epididymal white adipose tissue (eWAT), liver, and gastrocnemius muscle (GAS) from adult male mice. HSP90 is a loading control. Representative images from three independent repeats. d mRNA expression of Letmd1 from perinatal BAT from postnatal day 1 (P1), day 7 (P7), and day 14 (P14) male mice. nP1 = 4, nP7 = 5, nP14 = 5. Expression is normalized to Rpl32. e Western blots of LETMD1 and UCP1 proteins in BAT in the same sets as in (d). HSP90 is a loading control. Representative images from three independent repeats. f Representative western blots of LETMD1 and UCP1 proteins in BAT from adult male mice after 5 days of exposure to TN (thermoneutral, 30 °C), RT (room temperature, 23 °C), and cold (6 °C). α -Tubulin is a loading control. Representative images from three independent repeats. g mRNA expression of Letmd1 and thermogenic genes in the same sets as in (f). Expression is normalized to Rpl32. n = 3 per group. h Experimental scheme for a cold challenge in adult male mice. i H&E staining of BAT sections from mice exposed to cold (6 °C) and mice adapted to room temperature (23 °C) after cold stimulation. Scale bar, 200 μm. Representative images from three independent repeats. mRNA expression of Letmd1 (j) and Ucp1 (k) in the same sets as in (h). Expression is normalized to Rpl32. n = 3 mice per group. Data presented as mean ± SEM. *p < 0.05, **p < 0.005, ***p < 0.0005. The significance of the results was assessed using one-way ANOVA. Source data are provided as a Source Data file.
