Fig. 2: Combinatorial substrate synthesis by SPPS.
From: Discovery of extended product structural space of the fungal dioxygenase AsqJ
a Synthetic route towards N4-methylated benzo[1,4]diazepine-2,5-diones with different substitutions at the aromatic core, as indicated in blue. b Overall yields of the respective products 9/10 from 14 (over four steps). DCM dichloromethane, DIC N,N’-diisopropylcarbodiimide, DIPEA N,N-diisopropylethylamine, DMAP 4-dimethylaminopyridine, DMF N,N-dimethylformamide, FG functional group, Fmoc fluorenylmethyloxycarbonyl, HATU hexafluorophosphate azabenzotriazole tetramethyl uronium, HOAt 1-hydroxy-7-azabenzotriazole, TFA trifluoroacetic acid, THF tetrahydrofuran.
