Fig. 3: Effects of nomilin on the detoxifying gene expression in nhr-8 and daf-12 mutant C. elegans.

a, b Quantification of mRNA level of genes in nhr-8 (a) and daf-12 (b) mutant C. elegans. (two-tailed unpaired Student’s t-test, n = 3/each, each sample contains about 1000 worms). c–g Survival curve of nomilin in xenobiotic metabolism gene RNAi N2 C. elegans. Synchronised L1 worms were fed with E. coli (HT115) containing an empty control vector (L4440) until L4, then transferred to plates containing cyp35a3, gst-4, pgp-3 or pgp-14 RNAi HT115 with nomilin (50 μM) and DMSO (0.1%) as controls. All data were expressed as mean ± SEM. The detailed information is shown in Supplementary Table S4.