Fig. 4: Genetic variation in AtSUC8 affects protein function and is associated with different root–growth sensitivities to changes in substrate proton concentrations.
From: Single-gene resolution of diversity-driven overyielding in plant genotype mixtures
a Protein sequence alignments of natural SUC8 variants. Amino acid differences from Col-0 reference sequence are highlighted in red. b Sucrose transport activities of the Sav-0 and Uk-1 protein variants in oocytes. Different letters denote significant differences in Tukey’s post-hoc contrasts; n = 9, except Sav-0, 1 h where n = 10. c Primary root length differences of genotypes carrying either Sav-0 (S) or Uk-1 (U) alleles at the two loci (BRX and AtSUC8; number of RILs (n): BRXS = 38; BRXU = 42; AtSUC8S = 39; AtSUC8U = 33), grown on agarose plates with different substrate pH. Relative root length of different RILs carrying either allele at the BRX (right) or AtSUC8 locus (left); shown are log2-fold root-length differences of each RIL at pH 4.8 vs. 6.8 (e.g., a log-fold difference of –1 denoting roots being 2-fold shorter at pH 4.8 than at pH 6.8); *P = < 0.05 (ANOVA F1,74 = 5.8; P = 0.02); n.s. not significant. Boxes show interquartile ranges with medians; whiskers indicate data ranges up to 1.5 times the interquartile range from the box. Source data are provided as a Source Data file.
