Fig. 5: Hepatic ablation and overexpression of TIAL1 regulates cholesterol metabolism. | Nature Communications

Fig. 5: Hepatic ablation and overexpression of TIAL1 regulates cholesterol metabolism.

From: In vivo PAR-CLIP (viP-CLIP) of liver TIAL1 unveils targets regulating cholesterol synthesis and secretion

Fig. 5: Hepatic ablation and overexpression of TIAL1 regulates cholesterol metabolism.The alternative text for this image may have been generated using AI.

a Plasma from mice in (Fig. 3a), was fractionated by FPLC and lipoproteins were quantified through measurements of (a) cholesterol and (b) triglycerides in each fraction (n = 5 per group). c Selected fractions from (a, b) were subjected to western blot analysis of VLDL/IDL/LDL (ApoB48/100) as well as HDL (ApoA1) markers. d, e 35S counts from metabolic labeling and immunoprecipitation of TIAL1 targets and non-targets (controls, HUR, APOE) in primary mouse hepatocytes of WT and Tial1 LKO animals (n = 4 per group) (d) and their culture media (e). 35S counts from metabolic labeling and immunoprecipitation of TIAL1 targets and non-targets in primary mouse hepatocytes (n = 4 per group) (f) and the media (g) of Tial1 LKO animals, infected with Ad-GFP or Ad-Tial1. Data are presented as mean values ± SDs. Statistical significance was evaluated by two-tailed Student’s t-test. Source data are provided as a Source Data file.

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