Fig. 6: Hepatic inactivation of Tial1 reduces triglycerides and cholesterol secretion. | Nature Communications

Fig. 6: Hepatic inactivation of Tial1 reduces triglycerides and cholesterol secretion.

From: In vivo PAR-CLIP (viP-CLIP) of liver TIAL1 unveils targets regulating cholesterol synthesis and secretion

Fig. 6: Hepatic inactivation of Tial1 reduces triglycerides and cholesterol secretion.The alternative text for this image may have been generated using AI.

VLDL-TG and cholesterol secretion assays in 8-week-old (WT; n = 8, Tial1-LKO; n = 9) mice after 6 h fasting and intravenously injection of 500 mg/kg tyloxapol to block lipases. Plasma was taken at the indicated time-points and triglycerides (TG) (a) and total cholesterol (TC) levels (c) were determined by enzymatic assay. b VLDL-TG and d cholesterol secretion rates were calculated from the slopes of increased TG and TC for each mouse. e Plasma from mice fed a chow diet, 2% cholesterol diet (n = 6) for 3 days ad libitum or a high-fat diet for 8 weeks (n = 6) was fractionated by FPLC, and lipoproteins were quantified through measurements of cholesterol and triglycerides in each fraction. All data are presented as mean values ± S.D. Statistical significance was evaluated by two-tailed Student’s t-test (b, d) or two-way ANOVA with Holm-Šídâk post hoc analysis (a, c, e). Source data are provided as a Source Data file.

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