Fig. 4: Deletion of H69 and V70 is required for robust lung cell entry driven by  BA.4/BA.5 S protein. | Nature Communications

Fig. 4: Deletion of H69 and V70 is required for robust lung cell entry driven by  BA.4/BA.5 S protein.

From: Omicron subvariant BA.5 efficiently infects lung cells

Fig. 4: Deletion of H69 and V70 is required for robust lung cell entry driven by  BA.4/BA.5 S protein.

Cell entry mediated by mutated BA.2 and BA.4/BA.5 S proteins. Vero (A) and Calu-3 (B) cell entry was assessed by measuring the activity of virus-encoded firefly luciferase in cell lysates at 16–18 h after inoculation with particles containing the indicated S proteins. The average (mean) data ± SEM from three biological replicates (each with four technical replicates) are presented, with entry standardized against B.1 (set as 1). Statistical significance was analyzed by two-tailed Student’s t-tests with Welch correction (p > 0.05, not significant [ns]; p ≤ 0.05, *; p ≤ 0.01, **; p ≤ 0.001, ***), please see also Extended Data Table 4 and Supplementary Fig. 2.

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