Fig. 1: Morphometric changes of the NE upon ER stress and during recovery from ER stress.

a Schematic representation of the ER and the NE. The inset shows the LINC complex that spans the perinuclear space (PNS) between the INM and the ONM. NPC is a nuclear pore complex. b RT-TEM micrograph showing two representative MEF at steady state. Nucleoplasm (NP in the inset), cytoplasm (CP) and the double lipid bilayer (ONM and INM) constituting the NE are shown. c Upper panel showing a slice through a CET of the NE from a lamella through a MEF at steady state. Scale bar: 50 nm. The lower panel represents the isosurface representation of the corresponding segmented volumes for NE at steady state. ONM in salmon; INM in light purple. d Quantifications of corresponding ONM-INM distances at steady state (see Tomogram 14, Supplementary Fig. 1a). e Same as (b) during the perturbation of ER homeostasis with CPA. f Same as (c) under ER stress. Scale bar: 50 nm. g Same as (d) for a cell under ER stress (see Tomogram 1, Supplementary Fig. 1b). h Same as (b) in MEF recovering from ER stress (5 h after interruption of CPA exposure). i Same as (c), for a recovering MEF. Scale bar: 50 nm. Please also refer to Movie 1. j Same as (d) for a recovering MEF (see Tomogram 1, Supplementary Fig. 1c). k Same as (b) 48 h after interruption of the pharmacologic treatment. l Violin plot showing INM:ONM distances in five representative MEF examined by RT-TEM for each condition. The dotted line shows the average width of the PNS.