Fig. 1: Functional characterization of TPX2α5-α7.
From: Structural basis of protein condensation on microtubules underlying branching microtubule nucleation
a Domain organization of Xenopus laevis TPX2 with predicted alpha-helical regions and predicted intrinsic disorder. b Fluorescent images of GFP-tagged TPX2α5-α7 (1 µM) (green) co-localization with branched microtubule networks (red) in Xenopus egg extract. Data representative of three experimental replicates. c Fluorescent images of GFP-tagged TPX2α5-α7 (1 µM) (green) binding to GMPCPP-stabilized microtubules (red). Representative of three experimental replicates. d Gel filtration elution profile of monodispersed TPX2α5-α7 in the presence of 300 mM NaCl. e Fluorescent images of monodispersed GFP-tagged TPX2α5-α7 (5 µM) (green) with 300 mM NaCl, and in its co-condensed form with soluble tubulin (magenta) in BRB80 buffer alone. Experiments were repeated at least three times. f Fluorescent images of GFP-tagged TPX2α5-α7 (1 µM) (green) binding to GMPCPP-stabilized microtubules (red) in the presence of additional soluble tubulin (1 µM) (magenta). All scale bars, 10 µm. Experiments were repeated three times with similar results.
