Fig. 5: Inter-species conservation of human enhancers connected to orthologs between humans and pigs in each AT.
From: Dynamic chromatin architecture of the porcine adipose tissues with weight gain and loss
a Schematic of anatomic locations of AT depots in humans. b The number of PEIs in each AT. The number of genes/promoters are indicated above each bar. c Example evaluation of enhancer conservation in human ATs performed by mapping the contacts of PEIs from humans to pigs. In this case, PEIs of the IGFBP5 gene in human ASA are shown. According to the distance-normalized contact frequency (percentile score [PS]) (using C-intersecture software, see “Methods” for details), three types of enhancer conservation, including only sequence conserved (yellow), usage conserved (red), and sequence-specific (gray), are indicated in the magnified area. d Percent distribution of enhancers that are conserved in both sequence and usage for different types of orthologs between humans and pigs in each AT. The number of enhancers is shown in each bar. e Typical 1-1 ortholog examples (PPARG, CEBPB, IGFBP5, IL6ST, and VEGFA) contain enhancers that are highly conserved between humans and pigs. The conservation of enhancers for each gene is shown using humans (left) and pigs (right) as “reference” species, respectively. f Percentage distribution of enhancer conservation for 1-1 orthologs with significantly different changes in normalized expression between humans and pigs (5% confidence intervals) in each AT. The number of enhancers is shown in each bar. Source data for (b–f) are provided as a Source Data file.
