Fig. 4: Increased neuronal excitability in the Ank2-cKO cortex. | Nature Communications

Fig. 4: Increased neuronal excitability in the Ank2-cKO cortex.

From: Kv7/KCNQ potassium channels in cortical hyperexcitability and juvenile seizure-related death in Ank2-mutant mice

Fig. 4: Increased neuronal excitability in the Ank2-cKO cortex.The alternative text for this image may have been generated using AI.

a Normal resting membrane potential in Ank2-cKO SSC layer 2/3 pyramidal neurons (P19–22). (n = 12 neurons/8 mice [WT], 22,8 [cKO], Mann–Whitney test). b, c Increased input resistance in Ank2-cKO SSC layer 2/3 neurons (P19–22). (n = 10, 3 [WT], 12, 3 [cKO], two-way RM-ANOVA with Sidak’s test and Student’s t-test). d Increased current-firing curve slope in Ank2-cKO SSC layer 2/3 neurons (P19–22). (n = 7, 3 [WT], 9, 3 [cKO], two-way RM-ANOVA with Sidak’s test). e Diagram explaining mAHP. f Decreased mAHP amplitude in Ank2-cKO SSC layer 2/3 neurons (P19–22). (n = 16, 5 [WT], 21, 5 [cKO], Welch’s test). g Diagram explaining AP threshold and AP shape-related parameters. h–l Normal AP threshold and AP shape-related parameters in Ank2-cKO SSC layer 2/3 neurons (P19–22), as indicated by AP-threshold voltage and the height, width, and time-dependent voltage changes (dV/dt; maximal/depolarizing and minimal/repolarizing) of APs. (n = 13, 9 [WT], 18, 11 [cKO], Student’s t-test [AP threshold and Max dV/dt and min dV/dt], Mann-Whitney test [FWHM and amplitude]). The statistical tests involved two-sided analyses, and adjustments were made for multiple comparisons. Data are presented as mean values +/− SEM. P-values in figure panels: *p < 0.05, **p < 0.01, ***p < 0.001, ns, not significant.

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