Fig. 1: SCPSs is immunogenic and binds to RLP30.
a Ethylene accumulation in Arabidopsis Col-0 wild-type plants, rlp30-2 mutants, or an rlp30-2 line complemented with a p35S::RLP30-YFP construct 4 h after treatment with water (mock), 1 μM nlp20, or 1 µM P. pastoris-expressed SCPSs. b B. cinerea infected area as determined by lesion diameter on day 2 after 24 h-treatment of Col-0 wild-type plants, rlp30-2 mutants or the rlp30-2/RLP30-YFP complementation line with water (mock), 1 µM nlp20, or 1 µM SCPSs. c Bacterial growth in plants pre-treated with water (mock), 1 μM nlp20, or 1 μM SCPSs 24 h before infiltration of Pst DC3000. Bacteria (colony forming units, CFU) were quantified in extracts of leaves 3 days after inoculation. d Ethylene accumulation in plants of the Brassicaceae and Solanaceae family 4 h after treatment with water (mock), 1 μM flg22, or 1 µM SCPSs. e Ligand-binding assay in N. benthamiana transiently co-expressing SCPSs-myc and either RLP30-GFP or RLP23-GFP. Leaf protein extracts (Input) were used for co-immunoprecipitation with GFP-trap beads (IP:GFP) and immunoblotting with tag-specific antibodies. For a–d, data points are indicated as dots (n = 6 for a, b, d; n = 20 for c) and plotted as box plots (center line, median; bounds of box, the first and third quartiles; whiskers, 1.5 times the interquartile range; error bar, minima and maxima). Statistically significant differences from mock treatments in the respective plants are indicated (two-sided Student’s t test, **P ≤ 0.01, ***P ≤ 0.001). Source data are provided as a Source data file. All assays were performed at least three times with similar results.
