Fig. 5: RLP30 expression in N. tabacum confers increased resistance to bacterial, fungal, and oomycete pathogens.
a Ethylene accumulation in N. tabacum wild-type plants (Wt) or two transgenic lines (#49 and #55) stably expressing RLP30-RFP and AtSOBIR1-GFP after 4 h treatment with water (mock), 1 µM SCP from indicated sources, or 1.5 μg/ml SCP-likePsp. b Bacterial growth of P. syringae pv. tabaci in N. tabacum wild-type plants (Wt) or transgenic RLP30/SOBIR1 lines (#49 and #55). Bacteria (colony forming units, CFU) were quantified in extracts of leaves 3 days after inoculation. c B. cinerea infected area on leaves of N. tabacum wild-type plants (Wt) or transgenic RLP30/SOBIR1 lines (left, shown are representative leaves) and determination of lesion diameter on day 2 after drop inoculation (right). d Growth of Phytophthora capsici on leaves of N. tabacum wild-type plants (Wt) or transgenic RLP30/SOBIR1 lines by determination of lesion size (right) of lesions observed under UV light (left, shown are representative leaves) on day 2 after drop inoculation. Data points are indicated as dots (n = 6 for a; n = 20 for b, n = 10 for c, d) and plotted as box plots (center line, median; bounds of box, the first and third quartiles; whiskers, 1.5 times the interquartile range; error bar, minima and maxima). Statistically significant differences from wild-type (Wt) plants are indicated (two-sided Student’s t test, ***P ≤ 0.001). Source data are provided as a Source data file. Each experiment was repeated three times with similar results.
