Fig. 2: Design features impacting the resource load of synthetic constructs.

a Resource load of seven constitutive promoters and one inducible promoter (TETp, induced with 1 ng/µl). Increased promoter strength of the test plasmid leads to decreased capacity monitor levels in HEK293T (top) and CHO-K1 (bottom). b Resource load of the TET-ON system when induced with increasing dox concentrations in HEK293T (top) and CHO-K1 (bottom). c Resource load of different promoter-polyA combinations in HEK293T (left) and CHO-K1 (right). EGFP and mKATE mean RFU values for each pair are normalised to the construct bearing the same promoter in combination with SV40pA. The grey square indicates the UBp-HGHpA combination, which did not clone correctly and was left out of the analysis. d Resource load of different promoter-Kozak configurations in HEK293T (top) and CHO-K1 (bottom). EGFP output (left) and mKATE monitor levels (right) are shown for hACTB, EF1a and TET promoters. Values are reported as mean RFU ± std. Number of biological repeats for each sample is reported in Supplementary data file 3. Two sided Mann–Whitney test P-value: ****<0.0001, ***<0.0005, **<0.005, *<0.05. Exact P-values can be found in Supplementary data file 4. Data were analysed as described in the Methods section and Supplementary Note 1. Source data are provided in the Source Data file.