Fig. 3: NIb inhibits NPR1 from interacting with and being modified by SUMO3.
From: A plant RNA virus inhibits NPR1 sumoylation and subverts NPR1-mediated plant immunity
a Box and whisker plot with individual data points comparing the nuclear YFP signal intensity from the NPR1–SUMO3 interaction in the presence of SUMO1, NIb, or NIbsim2. The data are the mean of the nuclear signal intensity of 5 micrographs. The whisker indicates minimum and maximum, and the box indicates the 25th and 75th percentiles (edges of the box), and median (center line). The original figures are available in the Figshare repository under [https://doi.org/10.6084/m9.figshare.23243918]. b Influence of SUMO1, NIb, or NIbsim2 on the luciferase activity from the interaction between cLUC-SUMO3 and NPR1-nLUC. The high-low reference bar shows fluorescence signals, ranging from high (top) to low (bottom). c Immunoblots for NPR1 sumoylation in XVE::NIb 35S::NPR1-GFP seedlings after 1 mM SA treatment with or without 2 μM 17-β-estrogen. NPR1, NIb, and SUMO3 were detected with anti-GFP, anti-NIb, and anti-SUMO3 antibodies, respectively. d Competitive split-luciferase assay to evaluate the influence of NIb and GUS on the NPR1‒TGA3 interaction. e Phenotypes of mock- and TuMV-infected WT, 35S::NPR1-GFP (NPR1-GFP), 35S::sim3-GFP (sim3-GFP) and 35S:npr1sim3 seedlings at 18 dpi. f Bar chart of the relative TuMV-mCh genome amount in WT and transgenic plants at 18 dpi (n = 3). RT‒qPCR was performed with Actin II as the internal control, and the viral genome in TuMV-infected WT plants was normalized to 1. g Bar chart of relative PR1 expression level in mock or virus-infected WT, 35S::NPR1-GFP, 35S::sim3-GFP, and 35S:npr1sim3 plants at 48 hpi (n = 3). The expression of PR1 in TuMV-infected WT plants was normalized to 1. Data are presented as mean values ± SD. Statistical analyses were performed using Two-tailed Student’s t test. Source data are provided as a Source Data file.
