Fig. 3: Mutant ARPC5 biochemical studies. | Nature Communications

Fig. 3: Mutant ARPC5 biochemical studies.

From: Inherited ARPC5 mutations cause an actinopathy impairing cell motility and disrupting cytokine signaling

Fig. 3

a Western blotting analysis of patient 1 (P1), her parents, and healthy controls (HC) T-cell blasts lysates with antibodies specific to Arp2/3 complex subunits, as indicated. Vinculin and β-tubulin were used as loading controls. b Western blotting analysis of cell extracts from P1’s and HC’s fibroblasts after native gel electrophoresis. ARPC5- or ARPC2-specific antibodies were used to probe the Arp2/3 complex; GAPDH was used as a loading control. Blots in this figure are representative of at least two independent experiments. The numbers below the western blotting images (a and b) represent protein expression levels, quantitatively measured in relation to healthy controls, after normalization to the loading control. Healthy controls’ average value was set at 10. PAGE polyacrylamide gel electrophoresis. Source data are provided as a Source Data file.

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