Fig. 5: FRAP of PPP2R1A and NHSL1.
From: PPP2R1A regulates migration persistence through the NHSL1-containing WAVE Shell Complex
a B16-F1 cells transfected with GFP-PPP2R1A were subjected to photobleaching in the red area at time 0. Kymographs built from the yellow rectangle showing the recovery, with filtering for the width of the lamellipodium or the lamellipodial edge. b FRAP quantification. c Quantification of the time of half recovery and of the immobile fraction in the width of lamellipodia. Mean ± sem, n = 16 from three independent experiments. d B16-F1 cells were co-transfected with plasmids encoding GFP-NHSL1 and mScarlet-PPP2R1A. The two fluorescent proteins were subjected to simultaneous photobleaching in the red area at time 0. Kymographs built from the yellow rectangle show the recovery of the lamellipodial edge. e FRAP quantification. f Quantification of the time of half recovery and of the immobile fraction of both fluorophores. Student’s t test. Mean ± sem, n = 13 from three independent experiments. Statistical significance was calculated with two-tailed Mann–Whitney test and P values are indicated. Source data are provided as a Source Data file.
