Fig. 6: PPP2R1A depends on NHSL1 to regulate cell migration.
From: PPP2R1A regulates migration persistence through the NHSL1-containing WAVE Shell Complex
MCF10A cells were transfected with pools of siRNAs targeting PPP2R1A, NHSL1 or both. Non-targeting siRNAs were transfected as controls (CTRL). Western blots were used to validate depletion. a 2D cell trajectories and migration persistence of single MCF10A cells transfected with indicated siRNAs, Tracking 6 h, n = 23 cells. Three biological repeats with similar results, only one is displayed. Data are shown as mean ± SEM. Statistical significance was calculated with custom-made R programs and P values are indicated. b Haptotaxis of MCF10A cells along a gradient of fibronectin. Cell trajectories of MCF10A cells transfected with indicated siRNAs. Three biological repeats with similar results. Tracking 8 h, n = 81 cells. Mean ± 95% confidence intervals of Forward Motility Index are plotted. Trajectory plots are labeled in µm. Statistical significance was calculated with ordinary one-way ANOVA with post hoc Tukey’s multiple comparison test and P values are indicated. *P < 0.05; **P < 0.01; ****P < 0.0001. Source data are provided as a Source Data file.
