Fig. 2: TSPAN8 KO increases mucin secretion.

a Representative dot blot showing mucin-5AC·GFP secreted by WT and TSPAN8 KO cell lines. b Quantification of the amount of mucin-5AC·GFP in secreted fractions of WT and TSPAN8 KO cell lines. Each dot represents the signal from one secretion assay. Grouped in different colors are secretion assays that were processed in parallel. n = 9. Red dots represent the mean +/− the standard deviation. Statistical analysis was performed independently for basal and ATP-stimulated conditions. The p values of planned orthogonal contrasts are shown. c Lysates of WT and TSPAN8 KO cells of the secretion assay shown in a were processed for western blot analysis. d Representative dot blot of the total mucin-5AC·GFP in unstimulated WT and TSPAN8 KO cell lines. e Quantification of the amount of mucin-5AC·GFP in the cell lysates of WT and TSPAN8 KO cells. Each dot represents the signal from an independent sample. Grouped in different colors are samples that were processed in parallel. n = 9. Red dots represent the mean +/− the standard deviation. The p values of planned orthogonal contrasts are shown. f Lysates of WT and TSPAN8 KO cells of the samples shown in d were processed for western blot analysis. g Quantification of secreted mucin granules in WT and TSPAN8 KO cells during ATP stimulation. Each dot represents the average cumulative percentage of secreted granules. Values are expressed as proportional to time 0. The gray shadow represents the mean +/− the standard deviation. h Frames showing the lateral projection of a time-lapse of WT cells during ATP stimulation. Time is relative to the moment of ATP addition. Signal is from mucin5AC·GFP fluorescent emission and its intensity was color-coded in a pseudo-color look up table. Arrows point to mucin granules release. Arrowheads point to mucin granules inside cells before ATP stimulation. i Same as in h, but for TSPAN8 KO cells. Source data for a to g are provided as a Source Data file.