Fig. 5: Central memory phenotype induction in CXCR5^– and CXCR5⁺ IL-7Rα⁺CD4⁺ T cells.

a–c Purified OT-II cells were transferred into CD45.1⁺ SMARTA recipient mice followed by intranasal infection with PR8-OVA. Indicated donor cell populations at day 28 p.i. in the medLN were analyzed for CXCR6 and Ly6c staining (a) and Psgl-1 staining (b). c Indicated donor OT-II populations at day 28 p.i. in the medLN and spleen were analyzed for CD62L and CCR7 staining (n = 11). d Purified CD45.1⁺ OT-II cells were transferred into CD45.2⁺ C57BL/6 recipient mice followed by intranasal infection with PR8-OVA. Indicated donor OT-II populations at day 14 p.i. in the medLN were analyzed for CD62L and CCR7 staining (n = 3). e C57BL/6 mice were intranasally immunized with KLH-GP_61-80 and LPS. Indicated GP⁺ populations at day 35 p.i. in the spleen were analyzed for CD62L and CCR7 staining (n = 5). Data in a–e are representative (or pooled) results of at least two independent experiments. Bars represent average ±SD. The P-values were determined by a two-tailed unpaired t-test (b, d, e), or a one-way ANOVA with Tukey’s multiple comparisons test (c). Source data are provided as a Source Data file.