Fig. 2: Over-expression of Mt-DprE2 increases resistance to pretomanid and delamanid.
From: DprE2 is a molecular target of the anti-tubercular nitroimidazole compounds pretomanid and delamanid
The MICs of a pretomanid and b delamanid were established using M. bovis BCG strains containing the constitutive expression vectors pMV261 (red), pMV261-Mt-dprE1 (blue) and pMV261-Mt-dprE2 (green). c BTZ was used as a positive control for DprE1 inhibition; d isoniazid was used as a negative control for DprE1 and DprE2 inhibition. Cell viability was determined using the MABA and CFU enumeration. Arrows on the MABA graphs indicate the concentration of drug selected for CFU enumeration. Data were plotted and fitted using Prism GraphPad showing the percentage survival (MABA) or CFU/mL (individual data points shown as gray circles), based on the mean of triplicate data (n = 3) from independent experiments with bars representing the standard error. Source data are provided as a Source Data file.
