Fig. 2: Cryo-EM structures of oxymetazoline- and noradrenaline-bound Nb29–α1AAR-miniGsq and tamsulosin-bound α1AAR-Nb6 complexes.
From: Structural basis of α1A-adrenergic receptor activation and recognition by an extracellular nanobody
The cryo-EM density maps and structure models of the Nb29-α1AAR-miniGsq complexes bound to the agonists oxymetazoline (a, b) and noradrenaline (c, d), and α1AAR-Nb6 complex bound to the antagonist tamsulosin (e, f). The detergent micelle (a, c, e) and unmodelled mT4L (b) are shown in gray. The densities of the ligands (shown as sticks) are depicted as surfaces. Color code for the proteins is as follows: oxymetazoline-bound active α1AAR (blue), noradrenaline-bound active α1AAR (green), inactive α1AAR (orange), miniGsq (pink), Nb29 (yellow), and Nb6 (purple). Small molecules are colored as follows: oxymetazoline in magenta, noradrenaline in gray, and tamsulosin in cyan.
