Fig. 3: RNAseq analysis of iPSC-Heps co-cultured with M1 or M0 iPSC-Macs. | Nature Communications

Fig. 3: RNAseq analysis of iPSC-Heps co-cultured with M1 or M0 iPSC-Macs.

From: Modeling and therapeutic targeting of inflammation-induced hepatic insulin resistance using human iPSC-derived hepatocytes and macrophages

Fig. 3

a Principal component analysis of gene expression profile of iPSC-Heps after 24-h co-culture with M1 or M0 iPSC-Macs or in iPSC-Hep mono-culture (C, control). n = 3. b Venn diagram of significantly differentially expressed genes comparing the indicated conditions. n = 3, FDR-adjusted P value (P < 0.05) by Wald test. c Heatmap of the top 1000 differentially expressed genes in iPSC-Heps under the indicated conditions in three independent experiments. The Z-score represents the gene-wise deviation from the mean of the log-transformed and variance-stabilized read counts. n = 3. d Top 10 upregulated pathway clusters enriched (Cluster Enrichment Score > 1.3) in the genes differentially expressed between iPSC Heps co-cultured with M1 or M0 iPSC-Macs identified using DAVID. Vertical axis represents enrichment fold values and horizontal axis shows the names of GO-BP, GO-MF and GO-CC terms and KEGG pathways. Node color indicates the enrichment significance, red represents higher significance. n = 3, FDR-adjusted P value (P < 0.05) by Wald test. e Molecular activity predictor pathway analysis generated using IPA representing the regulatory effects with top consistency scores showing TNFα, IL1β and IFNγ as the pro-inflammatory cytokines most active on iPSC-Heps co-cultured with M1 iPSC-Macs. n = 3, FDR-adjusted P value (P < 0.05) by Wald test.

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