Fig. 5: Effect of TNFα and/or IL1β neutralization on inflammation and glucose metabolism changes in PHHs co-cultured with M1 PHMs. | Nature Communications

Fig. 5: Effect of TNFα and/or IL1β neutralization on inflammation and glucose metabolism changes in PHHs co-cultured with M1 PHMs.

From: Modeling and therapeutic targeting of inflammation-induced hepatic insulin resistance using human iPSC-derived hepatocytes and macrophages

Fig. 5

a, b Pro-inflammatory cytokine release into media (n = 4) (a) and western blot analysis of JNK phosphorylation (n = 3) (b) in PHHs after 24-h co-culture with M1 or M0 PHMs or in PHH mono-culture (C, control). Data are mean ± SD; one-way ANOVA (Dunnett’s test), *P < 0.05, **P < 0.01 and ***P < 0.001 vs. M1. c Gene expression analysis of PHHs after 24-h co-culture with M1 or M0 PHMs, in PHH mono-culture (C, control) or after 24-h co-culture with M1 PHMs including TNFα and IL1β neutralizing antibodies (M1+NAbs). Data are mean ± SD; JUN, NFKB2, TNF: n = 4, CASP1: n = 3, one-way ANOVA (Dunnett’s test), *P < 0.05 and ***P < 0.001 vs. M1. d–f Analysis of glucose release into 1 mM glucose-containing media 2 h after insulin (primary cells: n = 3, CW10030 iPSC-derived cells: n = 5) (d), western blot analysis of AKT phosphorylation 30 min after insulin (n = 3) (e) and gene expression analysis 1 h after insulin (primary cells: n = 3, CW10030 iPSC-derived cells: n = 4) (f) in hepatocytes after 24-h co-culture with M1 macrophages or in hepatocyte mono-culture (C, control) or after 24-h co-culture with M1 macrophages including TNFα and IL1β neutralizing antibodies (M1+NAbs). Primary cells, filled bars/symbols; CW10030 iPSC-derived cells, open bars/symbols. Data are mean ± SD; one-way ANOVA (Dunnett’s test), *P < 0.05 and **P < 0.01 vs. M1. Source data are provided as a Source Data file.

Back to article page