Fig. 5: SIVETs minimize host T cell exhaustion in tumors. | Nature Communications

Fig. 5: SIVETs minimize host T cell exhaustion in tumors.

From: Adoptive T cell transfer and host antigen-presenting cell recruitment with cryogel scaffolds promotes long-term protection against solid tumors

Fig. 5

a Schematic of the experiment. b Numbers of tumor-infiltrating T cells per mm3 of tumor volume. P-value was determined by two-tailed one-way ANOVA with Geisser–Greenhouse correction. Data are mean ± s.d. from n = 3 mice per condition for a single experiment. c, d Immunofluorescence imaging of T cells present in NT and SIVET_GMCSF (c), as well as specific T cell subtypes in TcellOnly_Depot and SIVET_GMCSF tumors (d). Images are tiled acquisitions from a slide selected from 30 continuous sections each from 1 mouse per condition. e Umap plots showing the expression of the indicated markers. f Umap density plots of individual treatment conditions showing distinct localization of cells based on treatment group. Denser (hot) regions indicate more cells. g Umap plot overlaid with Kmeans clusters of T cells in tumors. h Heatmap plot showing the average expression of the indicated T cell markers in each cluster after K-means analysis. i Heatmap plot showing the proportion of cells in each condition represented in each cluster. Some clusters of interest are highlighted. j Scatterplot comparing the relative enrichment levels of PD1+ LAG3− host CD8+ T cells (cluster 5), and PD1+ LAG3+ host CD8+ T cells (cluster 3) as a function of the treatment group. Data are pooled cells from n = 3 mice per condition for a single experiment.

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